5OYC

GH5 endo-xyloglucanase from Cellvibrio japonicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	55.004, 96.434, 158.954
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.450 - 1.600
R-factor	0.14046
Rwork	0.137
R-free	0.20386
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ZMR.PDB
RMSD bond length	0.018
RMSD bond angle	1.687
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.450	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.060
Rpim		0.695
Number of reflections	112339	5530
<I/σ(I)>	11.8	1
Completeness [%]	100.0	100
Redundancy	7.8	7.9
CC(1/2)	0.999	0.699

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	291	ammonium sulfate, MES buffer