5OYA
Unusual posttranslational modifications revealed in crystal structures of diatom Rubisco.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2015-11-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96863 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.170, 219.110, 220.230 |
| Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
| Resolution | 49.190 - 1.800 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mz2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.564 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.190 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.092 | 0.669 |
| Number of reflections | 470418 | |
| <I/σ(I)> | 6.58 | 1.49 |
| Completeness [%] | 96.1 | 94.9 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Tris pH 7.5, 5%PGA, 15%PEG4000 |
