5OY9
VSV G CR3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-26 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.28 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 122.350, 122.350, 197.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.850 - 3.600 |
| R-factor | 0.237 |
| Rwork | 0.236 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2J6J |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.819 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.700 | 3.690 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.286 | 5.305 |
| Rpim | 1.443 | |
| Number of reflections | 10749 | |
| <I/σ(I)> | 9.75 | 0.7 |
| Completeness [%] | 99.9 | 97.9 |
| Redundancy | 20 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% PEG 400, 50 mM Tris-HCl pH 8.5, 200 mM CaCl2, 0.2% dodecylmaltoside |
