5OY5
Monomeric crystal structure of RpBphP1 photosensory core domain from the bacterium Rhodopseudomonas palustris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-09-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 92.780, 92.780, 449.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 79.090 - 2.600 |
| R-factor | 0.19408 |
| Rwork | 0.192 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gw9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.011 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.000 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.680 | |
| Number of reflections | 34645 | 2758 |
| <I/σ(I)> | 8.5 | 1.7 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 11 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 4% PGA (poly-gamma-glutamic acid polymer), 200 mM KBr and 100 mM TrisHCl pH 8 |
