5OXV

Structure of the 4_601_157 tetranucleosome (C2 form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-11
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	348.839, 63.274, 271.361
Unit cell angles	90.00, 124.74, 90.00

Refinement procedure

Resolution	71.666 - 6.721
R-factor	0.3208
Rwork	0.317
R-free	0.35220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lz0
RMSD bond length	0.009
RMSD bond angle	1.472
Data reduction software	XDS (VERSION May 1, 2016 BUILT=20160617)
Data scaling software	Aimless (0.5.21)
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	200.000	7.510
High resolution limit [Å]	6.720	6.720
Rmerge	0.163	4.943
Number of reflections	9194	2554
<I/σ(I)>	5.8	0.3
Completeness [%]	100.0	100
Redundancy	6.5	7.4
CC(1/2)	0.980	0.164

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	291	30-60 mM KCl, 90-110 mM MgCl2 and 5 mM Na-cacodylate, pH 6