5OXI
C-terminally retracted ubiquitin L67S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979490 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 41.055, 48.813, 74.401 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.813 - 1.630 |
| R-factor | 0.1956 |
| Rwork | 0.193 |
| R-free | 0.23720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq aa 1-59 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.183 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.813 | 1.688 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.059 | 0.487 |
| Number of reflections | 19162 | 948 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 99.1 | 99.58 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 3 M ammonium sulfate, 0.1 M MES (pH 6.0); 2:1 protein to reservoir ratio |
