5OXC
Structure of Cerulean Fluorescent Protein at 1.02 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.010, 62.800, 69.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.720 - 1.020 |
| R-factor | 0.09972 |
| Rwork | 0.099 |
| R-free | 0.11466 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wso |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.245 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.730 | 1.050 |
| High resolution limit [Å] | 1.020 | 1.020 |
| Rmeas | 0.050 | 0.838 |
| Number of reflections | 114476 | 8333 |
| <I/σ(I)> | 19.51 | 2.06 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 6.03 | 5.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100mM HEPES 7.0, 12% PEG8000, 100mM MgCl2 |
