5OX8
Structure of Enhanced Cyan Fluorescent Protein at pH 5.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.07166 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.247, 62.624, 69.108 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.660 - 1.290 |
| R-factor | 0.13395 |
| Rwork | 0.132 |
| R-free | 0.16640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wso |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.712 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 1.360 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Rmeas | 0.036 | 0.880 |
| Number of reflections | 55928 | 5470 |
| <I/σ(I)> | 18.8 | 2 |
| Completeness [%] | 98.9 | 98 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 100mM citric acid pH 5.0, 12% PEG8000, 100mM MgCl2 |
