5OX5

HIF prolyl hydroxylase 2 (PHD2/ EGLN1) in complex with CCT6, a GSK1278863-related compound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9800
Spacegroup name	P 63
Unit cell lengths	110.147, 110.147, 39.368
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.690 - 2.251
R-factor	0.1761
Rwork	0.175
R-free	0.20480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bqx
RMSD bond length	0.003
RMSD bond angle	0.553
Data reduction software	HKL-2000 (706E)
Data scaling software	HKL-2000 (706E)
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.690	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.138	0.953
Rpim	0.064	0.449
Number of reflections	13170	1314
<I/σ(I)>	12.9	2.1
Completeness [%]	100.0	99.9
Redundancy	5.7	5.7
CC(1/2)	0.994	0.598

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	1.5 M sodium citrate tribasic dehydrate pH 6.5, Sitting drop (300 nl), protein-to-well ratio, 1:1.