5OW2
Japanese encephalitis virus capsid protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.928 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.310, 49.780, 68.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.320 - 1.980 |
| R-factor | 0.19026 |
| Rwork | 0.188 |
| R-free | 0.23731 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sfk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.428 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.320 | 2.030 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.058 | 0.862 |
| Rpim | 0.440 | 0.686 |
| Number of reflections | 11457 | 821 |
| <I/σ(I)> | 12.1 | 1.4 |
| Completeness [%] | 99.6 | 99 |
| Redundancy | 4.5 | 4.3 |
| CC(1/2) | 0.999 | 0.551 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 18% v/v 2-propanol, 0.1 M sodium citrate tribasic dihydrate pH 5.6, 16 % w/v polyethylene glycol 4,000. |
