5OVN
Crystal Structure of FIV Reverse Transcriptase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 103 |
| Detector technology | PIXEL |
| Collection date | 2017-04-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.968861 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 127.143, 127.143, 191.342 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 95.435 - 2.942 |
| R-factor | 0.2211 |
| Rwork | 0.219 |
| R-free | 0.26620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dol |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.967 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | BALBES |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 110.109 | 3.022 |
| High resolution limit [Å] | 2.942 | 2.942 |
| Rmerge | 0.100 | 1.178 |
| Total number of observations | 366187 | |
| Number of reflections | 37376 | 4577 |
| <I/σ(I)> | 17.1 | 2.2 |
| Completeness [%] | 99.9 | 99.76 |
| Redundancy | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 273 | 3.8 NaFormate, 0.2M AmFormate |
