5OV5
Bacillus megaterium porphobilinogen deaminase D82E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.160, 62.700, 91.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.340 - 1.810 |
| R-factor | 0.1957 |
| Rwork | 0.194 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mlv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.971 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.830 | 1.860 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.048 | 0.629 |
| Rmeas | 0.053 | 0.697 |
| Number of reflections | 26586 | 1907 |
| <I/σ(I)> | 20.7 | 2.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.7 | 5.8 |
| CC(1/2) | 1.000 | 0.766 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.7 | 294 | 0.2 M ammonium acetate, 0.1 M sodium acetate pH 3.7, 25% PEG 4000 |
