5OUH
Humanized alpha-AChBP (acetylcholine binding protein) in complex with lobeline.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-16 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.92818 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 87.785, 113.320, 146.964 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 89.740 - 2.500 |
R-factor | 0.2092 |
Rwork | 0.207 |
R-free | 0.24990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5afm |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | DIMPLE |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.740 | |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.141 | 2.890 |
Rmeas | 0.152 | 3.130 |
Rpim | 0.057 | 1.190 |
Number of reflections | 51511 | |
<I/σ(I)> | 12.7 | |
Completeness [%] | 100.0 | |
Redundancy | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH 7.5, 8% ethylene glycol and 10% PEG8000. |