5OU9

Crystal structure of Glycoprotein VI in complex with collagen-peptide (GPO)3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97242
Spacegroup name	P 41 21 2
Unit cell lengths	59.720, 59.720, 319.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.930 - 2.500
R-factor	0.22955
Rwork	0.228
R-free	0.26027
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ou7
RMSD bond length	0.008
RMSD bond angle	1.564
Data reduction software	iMOSFLM (1.0.7)
Data scaling software	Aimless (0.1.30)
Phasing software	PHASER (2.7.0)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.930	2.610
High resolution limit [Å]	2.500	2.500
Rmerge	0.113	0.971
Number of reflections	21273	2503
<I/σ(I)>	8.5
Completeness [%]	99.9	100
Redundancy	6.4	6.8
CC(1/2)	0.984	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	0.1 M MMT-buffer pH 9.0 25% (w/v) PEG1500