5OU8

Crystal structure of Glycoprotein VI in complex with collagen-peptide (GPO)5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97242
Spacegroup name	P 41 21 2
Unit cell lengths	59.970, 59.970, 313.212
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.300 - 2.500
R-factor	0.21051
Rwork	0.208
R-free	0.25739
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ou7
RMSD bond length	0.009
RMSD bond angle	1.635
Data reduction software	iMOSFLM (1.0.7)
Data scaling software	Aimless (0.1.30)
Phasing software	PHASER (2.7.0)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.300	2.610
High resolution limit [Å]	2.500	2.500
Rmerge	0.121	1.030
Number of reflections	20736	2463
<I/σ(I)>	9.7	1.8
Completeness [%]	99.5	99.8
Redundancy	7.9	7.4
CC(1/2)	0.995	0.535

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1 M MES pH 6.0 0.2 M MgCl2 20% (w/v) PEG-6000