5OU7

Crystal structure of the Glycoprotein VI loop truncation mutant PAVS-PAPYKN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99999
Spacegroup name	P 1 21 1
Unit cell lengths	78.650, 44.050, 117.660
Unit cell angles	90.00, 104.69, 90.00

Refinement procedure

Resolution	113.810 - 1.900
R-factor	0.22309
Rwork	0.221
R-free	0.25967
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gi7
RMSD bond length	0.011
RMSD bond angle	1.480
Data reduction software	iMOSFLM (1.0.7)
Data scaling software	Aimless (0.1.30)
Phasing software	PHASER (2.7.0)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	113.810	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.081	1.080
Number of reflections	58873	3656
<I/σ(I)>	5	1.1
Completeness [%]	95.1	93.3
Redundancy	2.1	2.1
CC(1/2)	0.990	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	0.1 M phosphate-citrate buffer pH 4.0 40% (v/v) PEG-300