5OU1
M. thermoresistible IMPDH in complex with IMP and Compound 1 (7759844)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2015-12-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 4 |
| Unit cell lengths | 88.720, 88.720, 84.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.360 - 1.780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.360 |
| High resolution limit [Å] | 1.780 |
| Number of reflections | 31464 |
| <I/σ(I)> | 10.95 |
| Completeness [%] | 100.0 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 100mM sodium acetate pH 5.5, 200mM calcium chloride, 8-14% iso-propanol |
