5OT4

Structure of the Legionella pneumophila effector RidL (1-866)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976250
Spacegroup name	C 1 2 1
Unit cell lengths	242.648, 230.909, 86.726
Unit cell angles	90.00, 90.40, 90.00

Refinement procedure

Resolution	167.270 - 3.000
R-factor	0.2321
Rwork	0.229
R-free	0.29490
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.545
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	167.270	167.270	3.190
High resolution limit [Å]	3.000	8.880	3.000
Rmerge	0.094	0.030	2.315
Rmeas	0.101	0.032	2.517
Total number of observations	651993
Number of reflections	93761	3671	14849
<I/σ(I)>	12.6	56.11	0.74
Completeness [%]	99.0	98.4	97.8
Redundancy	6.954	6.772	6.546
CC(1/2)	0.999	0.999	0.341

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	0.4 M sodium fluoride, 14-16% PEG 3350 0.1 M Bis-tris propane pH 7.5.