5OSN

Crystal Structure of Bovine Enterovirus 2 determined with Serial Femtosecond X-ray Crystallography

Replaces:  5MQU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SLAC LCLS BEAMLINE XPP
Synchrotron site	SLAC LCLS
Beamline	XPP
Temperature [K]	293
Detector technology	PIXEL
Collection date	2015-07-22
Detector	CS-PAD XPP
Wavelength(s)	1.305
Spacegroup name	F 2 3
Unit cell lengths	436.600, 436.600, 436.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.710 - 2.300
R-factor	0.233
Rwork	0.233
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bev
RMSD bond length	0.011
RMSD bond angle	1.600
Phasing software	CNS (1.3)
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.370
High resolution limit [Å]	2.300	2.300
Number of reflections	235593
<I/σ(I)>	5.4
Completeness [%]	82.1	83.6
Redundancy	2.3	2.4
CC(1/2)	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	1.500 M Ammonium Sulphate 0.100 M Tris