5ORI
Structure of caprine serum albumin in orthorhombic crystal system
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.270, 66.960, 213.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.300 - 1.940 |
R-factor | 0.19143 |
Rwork | 0.188 |
R-free | 0.24830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4luf |
RMSD bond length | 0.020 |
RMSD bond angle | 2.035 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.040 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.056 | 0.800 |
Number of reflections | 45413 | 5899 |
<I/σ(I)> | 18.77 | 2.02 |
Completeness [%] | 98.7 | 93.2 |
Redundancy | 5.17 | 4.38 |
CC(1/2) | 0.999 | 0.626 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Jeffamine ED2001, citric buffer pH 5.0, 0.02M BaCl2 |