5ORF

Structure of ovine serum albumin in P1 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.071060
Spacegroup name	P 1
Unit cell lengths	77.820, 78.050, 109.710
Unit cell angles	89.81, 74.54, 73.15

Refinement procedure

Resolution	49.000 - 2.540
R-factor	0.1969
Rwork	0.196
R-free	0.25758
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4luf
RMSD bond length	0.020
RMSD bond angle	2.119
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.540	2.540
Rmerge	0.092	0.694
Number of reflections	70636	7502
<I/σ(I)>	11.36	2.09
Completeness [%]	90.1	87.5
Redundancy	3.64	3.46

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20% PEG3350, 0.2 M ammonium chloride, 0.1 M proline