5OR7
Atomic structure of the murine norovirus protruding domain and sCD300lf receptor complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07252 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.110, 77.460, 140.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.825 - 2.046 |
| R-factor | 0.1822 |
| Rwork | 0.180 |
| R-free | 0.21680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lq6 5ffl |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.600 |
| Data reduction software | XDS (VERSION Nov 1, 2016) |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.980 | 2.120 |
| High resolution limit [Å] | 2.046 | 2.046 |
| Rmerge | 0.108 | 0.705 |
| Number of reflections | 52389 | |
| <I/σ(I)> | 11.45 | 2.31 |
| Completeness [%] | 99.5 | 97.5 |
| Redundancy | 5.5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 25% (w/v) PEG3000, 0.1 M sodium acetate (pH 4.6) |
