5OQQ

Crystal structure of the S. cerevisiae condensin Ycg1-Brn1 subcomplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	P 31 2 1
Unit cell lengths	185.534, 185.534, 148.321
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.254 - 2.790
R-factor	0.2059
Rwork	0.205
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5oqr
RMSD bond length	0.003
RMSD bond angle	0.527
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.440	2.940
High resolution limit [Å]	2.790	2.790
Rmerge	0.139	1.745
Rpim	0.046	0.564
Number of reflections	73424	10633
<I/σ(I)>	12.9	1.3
Completeness [%]	100.0	100
Redundancy	10.2	10.4
CC(1/2)	0.999	0.444

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	3% (w/v) PEG 4000, 0.1 M Na citrate pH 5.5 0.2 M Na acetate