5OQN

Crystal structure of the S. cerevisiae condensin Ycg1-Brn1 subcomplex bound to DNA (short kleisin loop)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	89.149, 116.220, 155.518
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.343 - 3.150
R-factor	0.2516
Rwork	0.249
R-free	0.28050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5oqp
RMSD bond length	0.003
RMSD bond angle	0.571
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.680	3.320
High resolution limit [Å]	3.150	3.150
Rmerge	0.198	1.115
Rpim	0.059	0.401
Number of reflections	28612	4129
<I/σ(I)>	8.4	1.9
Completeness [%]	100.0	100
Redundancy	12.3	8.6
CC(1/2)	0.999	0.287

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		280	4% (w/v) PEG 4000, 0.05 M Na citrate pH 5.6, 5 mM MgSO4