5OPF
Structure of LPMO10B from from Micromonospora aurantiaca
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-02 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.859, 55.088, 75.106 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.420 - 1.081 |
| R-factor | 0.1408 |
| Rwork | 0.140 |
| R-free | 0.15160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oy6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.921 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.420 | 1.120 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Number of reflections | 78485 | 7545 |
| <I/σ(I)> | 11.5 | 1.5 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 4.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.04 M potassium phosphate monobasic and 16 % w/v PEG8000 and 20 % v/v glycerol at a protein concentration of 21.9 mg/mL |
