5OOO
Structure of the Rift Valley fever virus NSs protein core domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 2.0 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 124.500, 124.500, 174.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 107.820 - 2.200 |
| R-factor | 0.2019 |
| Rwork | 0.201 |
| R-free | 0.21660 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.767 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.830 | 2.250 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.098 | |
| Number of reflections | 41684 | |
| <I/σ(I)> | 32.3 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | PEG 10000, NaCl, HEPES, DTT |
