5ONL
YNDL-apo (Zinc-free)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.612, 47.284, 99.893 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.750 - 1.700 |
| R-factor | 0.17563 |
| Rwork | 0.174 |
| R-free | 0.21218 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a9l |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.185 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.750 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 20566 |
| <I/σ(I)> | 22.75 |
| Completeness [%] | 99.0 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 1 Ammonium Sulphate, 0.15 M Sodium Citrate |
