5OND
RfaH from Escherichia coli in complex with ops DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.310, 43.190, 61.860 |
| Unit cell angles | 80.45, 75.48, 75.39 |
Refinement procedure
| Resolution | 41.542 - 2.100 |
| R-factor | 0.1889 |
| Rwork | 0.186 |
| R-free | 0.23340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oug |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.183 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.550 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 19931 | 2633 |
| <I/σ(I)> | 13.96 | 3.47 |
| Completeness [%] | 97.3 | 97.9 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 277 | protein:DNA molar ratio: 1:1 reservoir solution: 21 %(w/v) PEG monomethyl ether 550, 44.4 mM HEPES (pH 7.0), 4 mM MgCl2; drops: 2ul protein/DNA solution + 2 ul reservoir solution |
