5ONB
Drosophila Bag-of-marbles CBM peptide bound to human CAF40
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0396 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 105.570, 200.930, 59.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.730 - 3.000 |
| R-factor | 0.2182 |
| Rwork | 0.216 |
| R-free | 0.26790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fv2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 26082 | 1852 |
| <I/σ(I)> | 13.2 | 1.7 |
| Completeness [%] | 99.5 | 98.8 |
| Redundancy | 5.5 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 100 mM HEPES (pH 7.0) 200 mM CaCl2 15% PEG 6,000 |
