5OMT
Endonuclease NucB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.955, 59.955, 66.604 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.560 - 1.350 |
| R-factor | 0.15034 |
| Rwork | 0.148 |
| R-free | 0.18623 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.676 |
| Data scaling software | XDS |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.560 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 27361 | 1316 |
| <I/σ(I)> | 16.1 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.8 | 7.7 |
| CC(1/2) | 0.991 | 0.944 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MES pH6.5 12% PEG 20000 |
