5OMP
Human FKBP5 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 90.732, 90.732, 133.596 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 78.580 - 1.880 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5njx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS (VERSION Jun 1, 2017 BUILT=20170601) |
| Data scaling software | STARANISO (Version 1.7.4 (19-Jun-17)) |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 133.593 | 2.150 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 1.556 | |
| Rpim | 0.385 | |
| Number of reflections | 27180 | 1347 |
| <I/σ(I)> | 20.3 | 1.9 |
| Completeness [%] | 52.1 | 8 |
| Redundancy | 19.4 | 17 |
| CC(1/2) | 0.763 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 20 %w/v PEG MME 5K, 0.2 M NH4SO4, 0.1 M MES 6 pH |
