5OMN

GII.10 Vietnam 026 protruding domain in complex with Nanobody Nano-27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97319
Spacegroup name	P 62 2 2
Unit cell lengths	167.449, 167.449, 143.538
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.338 - 2.682
R-factor	0.2361
Rwork	0.235
R-free	0.26250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3onu 4x7e
RMSD bond length	0.002
RMSD bond angle	0.477
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.338	48.338	2.840
High resolution limit [Å]	2.680	7.950	2.680
Rmerge	0.150	0.036	1.958
Rmeas	0.154	0.037	2.010
Number of reflections	33589	1454	5175
<I/σ(I)>	20.6	68.13	1.65
Completeness [%]	99.5	99.2	97
Redundancy	19.296	16.115	19.341
CC(1/2)	0.999	0.999	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M sodium acetate