5OMI
Crystal structure of GP2 from Lassa virus in a post fusion conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97626 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 58.318, 103.433, 171.551 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.515 - 2.560 |
| R-factor | 0.2368 |
| Rwork | 0.231 |
| R-free | 0.28650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mko |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.935 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.520 | |
| High resolution limit [Å] | 2.560 | |
| Rpim | 0.029 | 0.661 |
| Number of reflections | 17135 | |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM BIS-TRS pH-6.5, 500 mM sodium potassium tartrate tetrahydrate, 6.0% PEG10000, 15 mM HCl and 3% diamino-pentane-dihydrochloride |
