5OLT
Crystal structure of the extramembrane domain of the cellulose biosynthetic protein BcsG from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2016-05-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.2782 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 80.658, 80.658, 97.673 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.850 - 1.450 |
| R-factor | 0.12812 |
| Rwork | 0.127 |
| R-free | 0.15715 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.556 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.850 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rpim | 0.023 | 0.206 |
| Number of reflections | 60090 | 8728 |
| <I/σ(I)> | 21.3 | 5 |
| Completeness [%] | 96.1 | 96.1 |
| Redundancy | 9 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | ammonium citrate, ZnCl2 |
