5OLS
Rhamnogalacturonan lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.420, 76.600, 122.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.380 - 2.200 |
| R-factor | 0.23506 |
| Rwork | 0.233 |
| R-free | 0.27105 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5olq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.381 |
| Data reduction software | xia2 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.700 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 22486 | 1650 |
| <I/σ(I)> | 13.4 | 1.6 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 6.9 | 7.2 |
| CC(1/2) | 0.998 | 0.553 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM potassium chloride and 20% PEG 3350 |
