5OLQ

Rhamnogalacturonan lyase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	77.550, 123.820, 138.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.650 - 1.480
R-factor	0.12883
Rwork	0.127
R-free	0.16890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ru4
RMSD bond length	0.012
RMSD bond angle	1.540
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.650	1.510
High resolution limit [Å]	1.480	1.480
Number of reflections	221195	10868
<I/σ(I)>	13.4	2.6
Completeness [%]	99.9	99.9
Redundancy	7
CC(1/2)	0.999	0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20 mM sodium/potassium phosphate 20% (w/v) PEG 3350