5OLD
X-ray structure of the adduct formed upon reaction of ribonuclease A with a tetranuclear Pt-thiosemicarbazone compound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-04 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.951, 32.584, 72.502 |
| Unit cell angles | 90.00, 89.35, 90.00 |
Refinement procedure
| Resolution | 72.500 - 1.780 |
| R-factor | 0.19827 |
| Rwork | 0.196 |
| R-free | 0.25043 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jvt |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.273 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.500 | 1.820 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.071 | 0.766 |
| Rpim | 0.047 | 0.750 |
| Number of reflections | 62971 | |
| <I/σ(I)> | 22.5 | 1.3 |
| Completeness [%] | 97.9 | 76 |
| Redundancy | 2.8 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 22% PEG4K 0.01 M sodium citrate pH 5.1 |
