5OL9

Structure of human mitochondrial transcription elongation factor (TEFM) N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0, 2.0664
Spacegroup name	P 43 21 2
Unit cell lengths	47.670, 47.670, 93.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.460 - 1.302
R-factor	0.1906
Rwork	0.190
R-free	0.19650
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	0.946
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.460	1.330
High resolution limit [Å]	1.300	1.300
Rmerge	0.105	4.570
Number of reflections	27069	1928
<I/σ(I)>	20.26	0.6
Completeness [%]	99.7	98.1
Redundancy	25.05	24.01
CC(1/2)	1.000	0.413

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	Sodium Acetate, Ammonium Acetate, PEG4000