5OL8
Structure of human mitochondrial transcription elongation factor (TEFM) C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0, 0.9765 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.480, 112.540, 88.840 |
| Unit cell angles | 90.00, 110.13, 90.00 |
Refinement procedure
| Resolution | 46.233 - 1.900 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.22780 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.506 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.233 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.051 | 2.490 |
| Number of reflections | 70199 | 4740 |
| <I/σ(I)> | 17.92 | 0.79 |
| Completeness [%] | 98.1 | 89.5 |
| Redundancy | 6.6 | 5.04 |
| CC(1/2) | 1.000 | 0.350 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Limited proteolysis with ArgC at RT for 20min. BIS-TRIS pH 5.5-6.5, MgCl2, PEG3350 |
