5OKU
R. palustris Rpa4515 with adipate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.900, 166.609, 82.175 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.650 - 2.070 |
| R-factor | 0.20965 |
| Rwork | 0.207 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qpq |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.893 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.650 | 2.110 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.630 | 2.590 |
| Rmeas | 0.670 | |
| Rpim | 0.210 | 0.900 |
| Number of reflections | 20144 | 1010 |
| <I/σ(I)> | 5 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.4 | 2.3 |
| CC(1/2) | 0.977 | 0.490 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 290 | ammonium sulphate, sodium acetate |
