5OKS
Non-conservatively refined structure of Gan1D, a 6-phospho-beta-galactosidase from Geobacillus stearothermophilus, in complex with 6-phospho-beta-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-01-01 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.260, 68.950, 153.010 |
| Unit cell angles | 90.00, 100.38, 90.00 |
Refinement procedure
| Resolution | 27.880 - 1.960 |
| R-factor | 0.1826 |
| Rwork | 0.181 |
| R-free | 0.21680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5okh |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.855 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.880 | 2.000 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.118 | 1.027 |
| Number of reflections | 70701 | 2275 |
| <I/σ(I)> | 6 | 1 |
| Completeness [%] | 89.6 | 45.6 |
| Redundancy | 3.7 | |
| CC(1/2) | 0.989 | 0.503 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 16-19% PEG 8K, 0.1M imidazole buffer pH 6.5, 3% MPD |
