5OKL
Human afamin monoclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.96598 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.915, 112.872, 109.228 |
| Unit cell angles | 90.00, 93.39, 90.00 |
Refinement procedure
| Resolution | 109.040 - 2.090 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vue |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.332 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.040 | 2.210 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.044 | 0.685 |
| Number of reflections | 66261 | |
| <I/σ(I)> | 13.59 | 1.67 |
| Completeness [%] | 89.6 | 78.8 |
| Redundancy | 2.9 | 2.8 |
| CC(1/2) | 0.990 | 0.702 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1.5 uL PEG 1k 30%, CH3COONH4 180mM, 3% 6-aminohexanoic acid plus 1.5 uL 5 mg/ml protein stock 20mM HEPES ph7.5 150 mM NaCl, microseeding |
