5OK6
Ubiquitin specific protease 11 USP11 - peptide F complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.770, 45.510, 100.610 |
| Unit cell angles | 90.00, 102.68, 90.00 |
Refinement procedure
| Resolution | 49.007 - 1.300 |
| R-factor | 0.1582 |
| Rwork | 0.157 |
| R-free | 0.17790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mel |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.346 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.1.29) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.010 | 49.010 | 1.330 |
| High resolution limit [Å] | 1.300 | 5.810 | 1.300 |
| Rmerge | 0.051 | 0.029 | 0.624 |
| Rmeas | 0.058 | 0.033 | 0.716 |
| Rpim | 0.028 | 0.016 | 0.345 |
| Total number of observations | 578764 | ||
| Number of reflections | 140611 | ||
| <I/σ(I)> | 16 | ||
| Completeness [%] | 98.3 | 98.5 | 96.6 |
| Redundancy | 4.1 | 4.1 | 4.1 |
| CC(1/2) | 0.999 | 0.998 | 0.762 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283.15 | 0.01M tri-sodium citrate, 16% PEG6000 |
