5OK6

Ubiquitin specific protease 11 USP11 - peptide F complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	65.770, 45.510, 100.610
Unit cell angles	90.00, 102.68, 90.00

Refinement procedure

Resolution	49.007 - 1.300
R-factor	0.1582
Rwork	0.157
R-free	0.17790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mel
RMSD bond length	0.015
RMSD bond angle	1.346
Data reduction software	XDS
Data scaling software	Aimless (0.1.29)
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.010	49.010	1.330
High resolution limit [Å]	1.300	5.810	1.300
Rmerge	0.051	0.029	0.624
Rmeas	0.058	0.033	0.716
Rpim	0.028	0.016	0.345
Total number of observations	578764
Number of reflections	140611
<I/σ(I)>	16
Completeness [%]	98.3	98.5	96.6
Redundancy	4.1	4.1	4.1
CC(1/2)	0.999	0.998	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283.15	0.01M tri-sodium citrate, 16% PEG6000