5OK1

D10N variant of beta-phosphoglucomutase from Lactococcus lactis trapped with native beta-glucose 1,6-bisphosphate intermediate to 1.9A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97948
Spacegroup name	P 21 21 21
Unit cell lengths	36.710, 74.490, 78.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.280 - 1.860
R-factor	0.20011
Rwork	0.198
R-free	0.24559
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wf5
RMSD bond length	0.011
RMSD bond angle	1.494
Data reduction software	XDS
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.280	1.890
High resolution limit [Å]	1.860	1.860
Rmerge	0.062	1.972
Rpim	0.018	0.558
Number of reflections	18807	933
<I/σ(I)>	25.8	1.2
Completeness [%]	100.0	99.9
Redundancy	12.9	13.3
CC(1/2)	1.000	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	291	24-34% PEG 4000 200mM Tris pH 7.5 200mM sodium acetate harvested after ca. 12 weeks