5OJR
YCF48 bound to D1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.320, 60.460, 103.460 |
| Unit cell angles | 90.00, 96.78, 90.00 |
Refinement procedure
| Resolution | 74.945 - 1.960 |
| R-factor | 0.1951 |
| Rwork | 0.193 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oj5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.109 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.950 | 1.990 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.140 | 1.061 |
| Number of reflections | 85525 | 4040 |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 99.3 | 95 |
| Redundancy | 3.8 | 3.5 |
| CC(1/2) | 0.995 | 0.499 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 20% v/v 2-Propanol, 20% w/v Polyethylene glycol 4000 |
