5OJH
Crystal structure of the extramembrane domain of the cellulose biosynthetic protein BcsG from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 80.444, 80.444, 97.529 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.670 - 1.550 |
| R-factor | 0.16746 |
| Rwork | 0.166 |
| R-free | 0.19831 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ab initio model from Rosetta |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.632 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.700 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rpim | 0.056 | 0.260 |
| Number of reflections | 53454 | 2609 |
| <I/σ(I)> | 15.3 | 3.3 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | ammonium citrate |
