5OJB
Structure of MbQ NMH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 |
| Unit cell lengths | 91.134, 91.134, 39.687 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.567 - 1.543 |
| R-factor | 0.2021 |
| Rwork | 0.201 |
| R-free | 0.23290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oj9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.935 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.073 | 1.285 |
| Number of reflections | 54004 | 8534 |
| <I/σ(I)> | 10.83 | 0.9 |
| Completeness [%] | 99.4 | 97.2 |
| Redundancy | 5.1 | 4.7 |
| CC(1/2) | 0.999 | 0.552 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M SPG (pH 7.0), 30% PEG1500 |
