5OJA
Structure of MbQ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.130, 51.900, 39.350 |
| Unit cell angles | 90.00, 98.06, 90.00 |
Refinement procedure
| Resolution | 38.961 - 1.347 |
| R-factor | 0.1863 |
| Rwork | 0.185 |
| R-free | 0.20650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oj9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.830 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.430 |
| High resolution limit [Å] | 1.347 | 1.347 |
| Rmerge | 0.044 | 0.475 |
| Number of reflections | 28974 | 4410 |
| <I/σ(I)> | 11.77 | 1.65 |
| Completeness [%] | 96.0 | 90.9 |
| Redundancy | 3.3 | 3.1 |
| CC(1/2) | 0.998 | 0.741 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M SPG (pH 7.0), 30% PEG1500 |
