5OJ8

Crystal structure of the KLC1-TPR domain ([A1-B5] fragment)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.978570
Spacegroup name	P 62 2 2
Unit cell lengths	69.530, 69.530, 199.730
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.250 - 2.247
R-factor	0.218
Rwork	0.216
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nf1
RMSD bond length	0.009
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.660	2.380
High resolution limit [Å]	2.247	2.247
Rmeas	0.066	1.524
Number of reflections	14301
<I/σ(I)>	21.25	1.1
Completeness [%]	98.6	97.5
Redundancy	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20 % PEG400, 0,15M Phosphate pH7.25 10% PEG10K