5OJ5
YCF48 bound to D1 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.730, 68.650, 56.190 |
| Unit cell angles | 90.00, 105.90, 90.00 |
Refinement procedure
| Resolution | 41.793 - 1.080 |
| R-factor | 0.1448 |
| Rwork | 0.144 |
| R-free | 0.16220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xbg |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.317 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.480 | 1.100 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rmerge | 0.083 | 0.874 |
| Rpim | 0.043 | 0.555 |
| Number of reflections | 149739 | 7286 |
| <I/σ(I)> | 10.7 | 1.8 |
| Completeness [%] | 97.2 | 95 |
| Redundancy | 3.6 | 3.3 |
| CC(1/2) | 0.986 | 0.518 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 298 | 0.2 M Ammonium Tartrate dibasic, 20% w/v PEG 3350 |
